SABIIA - Sistema Aberto e Integrado de Informação em Agricultura (Sabiia)

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	Info:eu-repo/semantics/other (2)
	Info:eu-repo/semantics/article (1)

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An Alternative and Rapid Method for the Extraction of Nucleic Acids from Ixodid Ticks by Potassium Acetate Procedure

BABT

Rodríguez,Islay; Fraga,Jorge; Noda,Angel Alberto; Mayet,Milagro; Duarte,Yanisia; Echevarria,Eduardo; Fernández,Carmen.

Four variants of the potassium acetate procedure for DNA extraction from ixodid ticks at different stage of their life cycles were evaluated and compared with phenol-chloroform and ammonium hydroxide methods. The most rapid and most efficient variant was validated in the DNA extraction procedure from the engorged ticks collected from bovine, canine as well as from house ticks for the screening of Borrelia burgdorferi sensu lato, Anaplasma spp. and Babesia spp. The ammonium hydroxide procedure was used for non-engorged ticks. All the variants were efficient and allowed obtaining PCR-quality material according to the specific amplification of 16S rRNA gene fragment of the original tick. DNA extracted from the ticks under the study was tested by multiplex PCR...

Tipo: Info:eu-repo/semantics/article

Palavras-chave: Ticks; PCR; DNA extraction.

Ano: 2014

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400542

Prevalence of antibodies to Borrelia burgdorferi sensu stricto in humans from a Cuban village

BJID

Rodríguez,Islay; Fernández,Carmen; Sánchez,Lizet; Martínez,Bárbara; Siegrist,Hans H.; Lienhard,Reto.

Lyme disease has not been officially reported in Cuba. However, clinical cases have been serologically reported. Seroprevalence survey of Borrelia burgdorferi sensu stricto antibodies in humans in the country has not been conducted. OBJECTIVE: To estimate the prevalence of borrelial antibodies in inhabitants of a village with historically high level of tick infestation. METHODS: Serum specimens from 247 persons randomly selected from the population of the village were examined by IgG Western blot using B31 strain for estimating the prevalence of antibodies profile. RESULTS: A seroprevalence value interval (95% CI) of 0.6%-7.2% was estimated for the studied population. The prevalent borrelial protein bands on immunoblots were 41, 72, 90/93, 34, 47, 60, 58,...

Tipo: Info:eu-repo/semantics/other

Palavras-chave: Borrelia burgdorferi; Prevalence; Lyme disease; Antibodies.

Ano: 2012

URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000100015

Serological evidences suggest Borrelia burgdorferi sensu lato infection in Cuba